u266 cell line  (ATCC)

Journal: Blood Research

Article Title: Hypermethylation-mediated silencing of RASD1 drives multiple myeloma pathogenesis

doi: 10.1007/s44313-026-00125-6

Figure Lengend Snippet: a MSP gel electrophoresis showing methylation (M) and unmethylation (U) status in representative samples. b RASD1 mRNA expression in U266 cells following DAC treatment. c Quantification of apoptosis rates (mean ± SD) in U266 cells (** P < 0.01). d Representative flow cytometry plots of apoptosis (Annexin V-YF647A/PI staining); Total apoptosis: 5.04% in the control group and 12.08% in the DAC-treated group

Article Snippet: The human MM cell line U266 was obtained from the American Type Culture Collection, and was maintained in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum.

Techniques: Nucleic Acid Electrophoresis, Methylation, Expressing, Flow Cytometry, Staining, Control

Journal: Cells

Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

doi: 10.3390/cells14221799

Figure Lengend Snippet: BBR decreases IgE secretion but not IgG secretion. The expression of IgE by IgE-producing myeloma cells, U266, was determined by ELISA following berberine (BBR) treatment at 10, 5, and 2.5 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) for 6 days ( A ). The expression of IgG by IgG-producing myeloma cells, ARH-77, was determined by ELISA, treated as U266 ( B ). N = 3–4, ** p ≤ 0.01, *** p ≤ 0.0001, ns = not significant. Each dot represents a single biological replicate.

Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Cells

Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

doi: 10.3390/cells14221799

Figure Lengend Snippet: BBR does not affect the viability of IgE or IgG producing cells. ( A ) The viability of IgE by IgE-producing myeloma cells, U266, was determined by trypan blue exclusion assay, following berberine (BBR) treatment at 10, 5, and 2.5 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) for 6 days. ( B ) The same was performed for IgG-producing myeloma cells, ARH-77. ( C ) Total cell count determined by Trypan Blue was used to determine total cells per mL of U266 cells and ( D ) ARH-77 cells. N = 3. BBR= Berberine. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

Techniques: Trypan Blue Exclusion Assay, Concentration Assay, Cell Counting

Journal: Cells

Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

doi: 10.3390/cells14221799

Figure Lengend Snippet: BBR affects proliferation of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) The proliferation of IgE by IgE-producing myeloma cells, U266, was determined by XTT assay, following berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) for 6 days. ( B ) The same was performed for IgG-producing myeloma cells, ARH-77. N = 3. BBR = berberine, DMSO = dimethyl sulfoxide. * p ≤ 0.05, ns = not significant. Each dot represents a single biological replicate.

Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

Techniques: XTT Assay, Concentration Assay

Journal: Cells

Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

doi: 10.3390/cells14221799

Figure Lengend Snippet: BBR affects cell cycle genes of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) qRT-PCR of IgE heavy chain ( IGHE ) and cell cycle genes cyclin D1 ( CCND1 ), tumor protein 53 ( TP53 ), and growth arrest and DNA-damage inducible alpha ( GADD45A ), in IgE-producing U266 were performed after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL). qPCR analysis of cell cycle proteins of IgE-producing U266 was performed after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL) and data were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) expression. ( B ) The same was performed for ARH-77, with analysis of IgG heavy chain ( IGHG ). N = 3. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

Techniques: Quantitative RT-PCR, Concentration Assay, Expressing

Journal: Cells

Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

doi: 10.3390/cells14221799

Figure Lengend Snippet: BBR affects cell cycle genes of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) Western Blot analysis of cell cycle genes in IgE-producing U266 was performed after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL). ( B ) The same was performed for ARH-77. N = 3. CDK2 = cyclin dependent protein kinase 2, CDKN1C = cyclin dependent kinase inhibitor 1C, TP53 = tumor protein 53. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

Techniques: Western Blot, Concentration Assay

Journal: Cells

Article Title: A Small Molecule Compound, Berberine Reduces IgE but Not IgG Production via Promoting miRNA-34a-p53 Axis

doi: 10.3390/cells14221799

Figure Lengend Snippet: BBR affects expression of miRNAs involved in modulation of the p53 pathway of IgE-producing myeloma cells with no effect on IgG-producing myeloma cells. ( A ) miRNA isolation and qPCR analysis of miRNAs (miR) implicated IgE-regulation, miR-155, miR-143, miR-34a, and miR-146a were performed on U266 after 6 days of berberine (BBR) treatment at 10 μg/mL compared to the equivalent concentration of DMSO (0 μg/mL). ( B ) The same was performed for ARH-77. N = 3. * p ≤ 0.05, ** p ≤ 0.01, ns = not significant. Each dot represents a single biological replicate.

Article Snippet: The IgE-producing human myeloma U266 cell line and IgG-producing human myeloma ARH-77 cell lines were obtained from American Type Culture Collection, (ATCC, Rockville, MD, USA).

Techniques: Expressing, Isolation, Concentration Assay